42 research outputs found
Extensive and Intimate Association of the Cytoskeleton with Forming Silica in Diatoms: Control over Patterning on the Meso- and Micro-Scale
BACKGROUND: The diatom cell wall, called the frustule, is predominantly made out of silica, in many cases with highly ordered nano- and micro-scale features. Frustules are built intracellularly inside a special compartment, the silica deposition vesicle, or SDV. Molecules such as proteins (silaffins and silacidins) and long chain polyamines have been isolated from the silica and shown to be involved in the control of the silica polymerization. However, we are still unable to explain or reproduce in vitro the complexity of structures formed by diatoms. METHODS/PRINCIPAL FINDING: In this study, using fluorescence microscopy, scanning electron microscopy, and atomic force microscopy, we were able to compare and correlate microtubules and microfilaments with silica structure formed in diversely structured diatom species. The high degree of correlation between silica structure and actin indicates that actin is a major element in the control of the silica morphogenesis at the meso and microscale. Microtubules appear to be involved in the spatial positioning on the mesoscale and strengthening of the SDV. CONCLUSIONS/SIGNIFICANCE: These results reveal the importance of top down control over positioning of and within the SDV during diatom wall formation and open a new perspective for the study of the mechanism of frustule patterning as well as for the understanding of the control of membrane dynamics by the cytoskeleton
High plasma uric acid concentration: causes and consequences
High plasma uric acid (UA) is a precipitating factor for gout and renal calculi as well as a strong risk factor for Metabolic Syndrome and cardiovascular disease. The main causes for higher plasma UA are either lower excretion, higher synthesis or both. Higher waist circumference and the BMI are associated with higher insulin resistance and leptin production, and both reduce uric acid excretion. The synthesis of fatty acids (tryglicerides) in the liver is associated with the de novo synthesis of purine, accelerating UA production. The role played by diet on hyperuricemia has not yet been fully clarified, but high intake of fructose-rich industrialized food and high alcohol intake (particularly beer) seem to influence uricemia. It is not known whether UA would be a causal factor or an antioxidant protective response. Most authors do not consider the UA as a risk factor, but presenting antioxidant function. UA contributes to > 50% of the antioxidant capacity of the blood. There is still no consensus if UA is a protective or a risk factor, however, it seems that acute elevation is a protective factor, whereas chronic elevation a risk for disease
Going with the Flow: Detection of Drift in Response to Hypo-Saline Stress by the Estuarine Benthic Diatom Cylindrotheca closterium
Avoidance response is a well-known mechanism for escaping environmental stress. For organisms with reduced
active movement, such as benthic microalgae, drifting could be a specifically selected mean of avoiding less
favorable environments. To test this hypothesis, a system was developed to assess if hypo-saline stress triggers drift
in the estuarine benthic diatom Cylindrotheca closterium. Concurrently, the effects of salinity on growth inhibition
were also investigated in order to compare the sensitivity of this endpoint with the drift response, and to estimate the
immediate population decline caused by both drift and population growth responses. It was verified that the salinity
value that inhibited the algal population growth by 50% (IGS50) was 19, while the salinity value that triggered the drift
response by 50% of the population (TDS50) was 15. These results indicate that drift is an identifiable response
triggered to escape stressful environments. The combination of the two responses (population growth and drift)
showed that population decline based exclusively on the inhibition of population growth may result in an
underestimation of the risk, compared with the decline when drifting to avoid stress is also taken into account.This study was partially funded by the “Fundação para a Ciência e a Tecnologia” (FCT, Portugal) through a postdoctoral fellowship (reference
SFRH/BPD/74044/2010) to C.V.M. Araújo, through the SALTFREE project (contract PTDC/AAC-CLI/111706/2009) and through “Ciência 2007 - Human
Potential Operational Program” (POPH) and “Quadro de Referência Estratégico Nacional” (QREN) through the European Social Fund (ESF) and the
Spanish Ministry of Education and Science (MEC) funds